EMBL Events’ Post

Highlights from EMBL 'Quantitative biology to molecular mechanisms' day 2: ▶️ Alexander Van Oudenaarden showed how to detect rare variants in mitochondrial DNA in single cells, how to mutate it, and that it mostly presents a neutral drift. Checking membrane potentials, however, revealed phenotypes. ▶️ Helge Grosshans presented a very cool integration of ATAC-seq, TF-binding motifs and peak phases to explain rhythmic chromatin states in nematode development. ▶️ Mo Khalil, Luca Giorgetti and Christine Moene all showcased very exciting reporter essays to understand TF-binding and its modulation through cooperativity, enhancer-promoter interactions and 3D chromatin. 3 take-aways: reduce binding affinity and increase cooperativity to recude off-target effects (MK), neutral TADs are a great playground (LG) and, surprisingly, target gene-body and UTRs are important for enhancer effects (CM). ▶️ Marko Jovanovic presented SPIDR: a multiplexed method for mapping RNA-Protein interactions. The trick - beads with barcode plus split and pool: https://lnkd.in/dHPEcW75 Also, as always, a great poster session! Presented our work on gene-expression based cellular lineage inference, and learned about cell segmentation for image-based approaches and how machine learning can come up with equations to estimate over-complex functions. #EMBLOmics EMBL Events